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. 2017 Feb 13;17:14. doi: 10.1186/s12894-017-0203-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Detection of immunocasp-3 protein secreted by genetically modified Jurkat cells. a: Genomic DNA was isolated from the immunocasp-3-modified Jurkat cell clones and analyzed by PCR: J591 (lane 1); revcaspase-3 (lane 2); β-actin (lane 3), respectively. b: Western blot analysis of the concentrated cell culture medium obtained from Jurkat cells stably transfected with the immunocasp-3 gene. Blots were probed with the anti-caspase-3 antibody. c: Growth and proliferation characteristics of Jurkat cells transfected with immunocasp-3 or unmodified Jurkat cells