Fig. 5.

Buformin diet during the premalignant risk window hinders mammary morphogenesis and proliferation in MMTV-erbB-2 mice. Mice were fed control and buformin diets for 10 weeks (N = 6). At 18 weeks of age, the mice were sacrificed for whole mount preparation of mammary glands (a). Representative images of H&E stained (b) and Ki67 immunostained (c) mammary tissues from 18-week-old mice are shown. Brown staining in C indicates Ki67⁺ cells, from which the percentage of Ki67⁺ cells from each group is graphed in d. All values are depicted in the graph as the mean ± S.E. (**p < 0.01)