Fig. 2.

Relationship of ALK expression with Sox11 and N-myc in UCSs. a Various ALK promoter constructs used in this study. b Ishikawa cells were transfected with ALK promoter constructs, together with the indicated Sox genes. Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means ± SDs. The experiment was performed in duplicate. c Ishikawa cells were transfected with various ALK promoter constructs, together with Sox11. d Left: the shortest ALK reporter constructs, along with either c-myc or N-myc, were transfected into Ishikawa cells. Right: various promoter constructs were used for evaluating transcriptional regulation of the ALK promoter by N-myc. e Left: Ishikawa cells were transfected with N-myc promoter constructs, along with Sox11. Right: analysis of mRNA levels for the N-myc gene by real time RT-PCR in Ishikawa cells after transfection of Sox11. f Ishikawa cells were transfected with ALK promoter constructs, together with Sox11 and N-myc