Fig. 5.

Inhibition of apoptosis by ALK/pAkt axis. a After treatment of Hec251 cells stably overexpressing exogenous ALK (H251-ALK#16) with doxorubicin (Dox) for 48 h, cells undergoing apoptosis (sub-G1) were detected by flow cytometry. The experiments were performed in triplicate, using independent samples. b Analysis of protein expression levels by western blot assay in the stable cells after treatment with Dox for 48 h. c Values of endogenous pAkt relative to tAkt protein (upper) and endogenous bcl2 relative to bax protein (lower) were calculated by normalization to β-actin using NIH ImageJ software