Table 1.
Primer sequences used in the study
| Assay | Gene/region | Sequence | |
|---|---|---|---|
| Promoter | ALK | −2056 Forward | 5'-GCTCGCTAGCCTCGAACTGTGTGATGTGTTAG-3' |
| −1456 Forward | 5'-GCTCGCTAGCCTCGATGAGATAATTCTTTG-3' | ||
| −956 Forward | 5'-GCTCGCTAGCCTCGATGAGTTCTGTGTTGG-3' | ||
| −416 Forward | 5'-GCTCGCTAGCCTCGAAGTCGGACCCGTTTA-3' | ||
| −146 Forward | 5'-GCTCGCTAGCCTCGAAGGCCGGACTGCGTG-3' | ||
| +30 Reverse | 5'-TCTTGATATCCTCGAGTACCAGCTGCTACC-3' | ||
| N-myc | −221Forward | 5'-CTCGCTAGCCTCGCAGCAGCTTTGCAGCCTTCTC-3' | |
| +1312 Reverse | 5'-AACCAGGTTCCCCAATCTTC-3' | ||
| Twist 1 | −1086 Forward | 5'-GCGTATCCAAGCATTTGGAATTGGGG-3' | |
| −601Foward | 5'-CCCAGGACCTCCGGGCTGGG-3' | ||
| −101 Forward | 5'-GGGGACTGGAAAGCGGAAAC-3' | ||
| +101 Forward | 5'-GCGTCCAGCCGTTGGGCGCT | ||
| +350 Reverse | 5'-CTCTCGAGCGGCGACGCGTGGCCTC-3' | ||
| Mutagenesis | ALK E-box1 | Forward | 5'-GCTGTATAGTGGCGGGCGCCCAGGCAG-3' |
| Reverse | 5'-GCCCGCCACTATACAGCTGGCTGAGCCGCGC-3' | ||
| ALK E-box2 | Forward | 5'-CAGGTATAGTGCGATCCAGCGGCTCTG-3' | |
| Reverse | 5'-GGATCGCACTATACCTGGGCGCCCGCCACTT-3' | ||
| shRNA | sh2969 | Forward | 5'-GATCCCGAATATTAAGCATTATCTAAAGCT |
| TCCTGTCACTTTAGATAATGCTTAATATTCTTTTTTG-3' | |||
| Reverse | 5'-AATTCAAAAAAGAATATTAAGCATTATCTA | ||
| AAGTGACAGGAAGCTTTAGATAATGCTTAATATTCGG-3' | |||
| sh2386 | Forword | 5'-GATCCCGTACAAACCAGTTAATCCAGAGCT | |
| TCCTGTCACTCTGGATTAACTGGTTTGTACTTTTTTG-3' | |||
| Reverse | 5'-AATTCAAAAAAGTACAAACCAGTTAATCCA | ||
| GAGTGACAGGAAGCTCTGGATTAACTGGTTTGTACGG-3' | |||
| ChIP | ALK | −126 Forword | 5'-GCGGAGTTGGCTTGTGAGCC-3' |
| +12 Reverse | 5'-TGCTACCACCGCTGCCGCC-3' | ||
| Twist 1 | −101 Forward | 5'-GGGGACTGGAAAGCGGAAAC-3' | |
| +62 Reverse | 5'-TGCAGAGCCCGCGAGGTGT-3' | ||
| mRNA | Twist1 | Forward | 5'-ATGATGCAGGACGTGTCCAGC-3' |
| Reverse | 5'-CTAGTGGGACGCGGACATGG-3' | ||
| N-myc | Forward | 5'-TTCTCACGCTCAGGGACCACGT-3' | |
| Reverse | 5'-GAAGCGTCTAGCAAGTCCGAGC-3' | ||
| Slug | Forward | 5'-ACGCAATCAATGTTTACTCG-3' | |
| Reverse | 5'-TGAAGAGAAAGGTTACTGTC-3' | ||
| Snail | Forward | 5'-TGCCTCGACCACTATGCCGC-3' | |
| Reverse | 5'-AGCATTGGCAGCGAGGCGGT-3' |