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. 2017 Feb 13;17:11. doi: 10.1186/s12896-017-0336-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Transfection and localization of Oct4, Sox2, Klf4 and c-Myc in GEFs. Indirect immunofluorescence staining was used to detect the transfection of mRNA from the four reprogramming factors Oct4, Sox2, Klf4 and c-Myc. After five consecutive transfections, expression of the four reprogramming factors were observed in GEFs, and localized in the nucleus. GEFs without transfection were used as the blank control. Immunofluorescence staining without primary antibodies was employed as the negative control. Neither blank control or negative control showed positive staining. Scale bar: 50 μm