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. 2017 Feb 13;17:11. doi: 10.1186/s12896-017-0336-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Reprogramming mechanism in the process of mRNA induction. a The expression of Oct4, Sox2, Klf4, and c-Myc were evaluated by qRT-PCR. Their expression reached the maximum 6 days after transfection and then decreased to the minimum on day 12. Then, the expression of these reprogramming factors increased gradually afterwards. b Expression of the endogenous pluripotent genes Nanog and TERT began to increase on day 12. c Western blot analysis of the OCT4, SOX2, KlLF4, C-MYC and NANOG during mRNA-induction process. d1, d6, d9, d12, d15, d18, d21 represent the day after exogenous mRNA transfection into GEFs. d Schematic diagram of the endogenous and exogenous expression pattern of the reprogramming markers during mRNA-induced GEFs reprogramming process