Fig. 6.

Epigenetic and genetic changes of goat iPSCs. a Bisulfite genomic sequencing of the key pluripotent gene Nanog promoter area showed reduced methylation level during goat iPSCs generation, and the low methylation status was maintained in goat iPS1 passages from 1 to 18. The open and closed circles represent the unmethylated and methylated CpGs, respectively. GEFs without transfection was used as the negative control b Karyotype analysis of the goat iPS1 cell line at passages 8 and 18 showed normal 58 XY karyotypes