Fig. 7.

Analysis of goat Sox2 promoter. a Goat Sox2 promoter activity was assessed by dual luciferase reporter assay of different promoter deletions, and the PGL3-P2 deletion showed the highest activity among all the deletions we colned. Loss the function of AP-2 and Elf-1 transcription factor binding sites significantly reduced the promoter activity. Treatment with TSA and 5-Azadc significantly improved the activity of PGL3-P2. (* represents for P value <0.05, and ** represents for P value <0.01) b Bisulfite genomic sequencing of the Sox2 promoter showed 31 modified CpG sites, among which, the methylated sites were significantly reduced in derived goat iPSCs