Fig. 2.

Basic plasmid library for E. coli. The plasmid library consists of 13 donor plasmids, resulting in 27 different expression plasmids. Three promoters (T7/lac, T5/lac and araBAD) were combined with a His₆ tag and either GST or Trx as a fusion partner (or no fusion partner). A thrombin cleavage site was also included in the constructs with a fusion partner. The library includes three AMP genes encoding IMPI, BR021 or the L. sericata antifungal peptide (AFP). The T7 transcriptional terminator was present in all constructs. The chosen BsaI overhangs are shown above the elements