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. 2017 Feb 13;16:29. doi: 10.1186/s12934-017-0637-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Basic plasmid library for P. pastoris. The plasmid library consists of 11 donor plasmids, resulting in 18 different expression plasmids. Two promoters (pGAP and pAOXI) were combined with three secretion factors (the α-mating factor, Kex2, DDDK), a His₆ tag and an intein sequence. The tAOXI transcriptional terminator was present in all constructs