Figure 2.

Effects of Hibiscus syriacus L. flower (HSF) absolute on migration in HaCaT cells. The cells were stimulated with recombinant human epidermal growth factor (EGF; 5 ng/mL) or HSF absolute (0.001–0.1 mg/mL) for 3 h. Migration was examined using a Boyden chamber assay. (a) The membrane was stained using Diff-Quick solution and then images were obtained using microscopy (× 200). (b) Migration in the quiescent state is expressed as 100% (n = 4). EGF was used as a positive control. Data are expressed as the mean ± SE. *P value less than 0.05 versus nonstimulated group.