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. 2017 Feb 14;7:42433. doi: 10.1038/srep42433

Figure 1. LS3 alters BK channel function in wild type or humanized C. elegans.

Figure 1

(A) Schematic of LS3 shows the sequence, 1–9 disulfide bridge and two enriched motifs (purple and red residues, enriched ~4000 and ~3000 fold, respectively, during the selection process). (B) Bar graph of crawl speed normalized to vehicle-treated controls shows that LS3 reduced crawl speed for wild-type (gray bars) but not slo-1 null (black bars) worms in a concentration dependent manner (WT vs. slo-1(null) at 250 and 750 μM: p < 0.05 and p < 0.001, N = 139–372, Two-way ANOVA with Dunn’s post-hoc correction). (C) Bar graph of crawl speed normalized to vehicle-treated controls shows that reduction in crawl speed by 750 μM LS3 was rescued on the slo-1 null background with extrachromosomal expression of either the worm (slo-1(+)) or the human (hslo(+)) BK channel gene (vs. slo-1(null): p < 0.001 for both, N = 78–162, Two-way ANOVA with Dunn’s post-hoc correction). Worms were bathed in LS3 or vehicle for 30 min prior to tracking crawl speed.