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. 2017 Feb 14;7:42433. doi: 10.1038/srep42433

Figure 2. LS3 alters the gating of human BK channels.

Figure 2

Human BKα channels (ZERO isoform) expressed in HEK293 cells were recorded in inside-out patches. Peptide was applied by diffusion to the extracellular side. Intracellular calcium held at 750 nM. Each trace is 3 seconds long. (A,B) Single channel traces show 500 nM LS3 reduces the probability of opening at 60 mV (A) and 100 mV (B). (C–E) Bar graphs displaying the post-peptide Po relative to the starting Po across LS3 concentrations. At 500 pM through 500 nM LS3 reduced the Po at each holding potential (pre vs. post: *p < 0.05, **p < 0.01, ***p < 0.001, N = 6–12, planned Student’s t-tests). 50 pM LS3 did not significantly alter the Po.