Figure 2. Effect of sirtinol treatment on root meristem size and cell division.

(a) Sirtinol treatment reduced the root meristem size. To analyze root meristem size, number of cortical cells was quantified by counting from QC to first elongating cell (marked by white arrow heads) in control and sirtinol treated seedling at 2 dag. Scale bar: 100 μm. (b) Number of cortical cells is reduced in roots of sirtinol treated seedlings. Error bars indicate ± standard deviation (SD) (n = 20). One-way ANOVA was performed for statistical analysis. Asterisks indicate significant statistical differences, ***P < 0.001, **P < 0.01, *P < 0.05. Experiment was repeated 3 times with reproducible results. (c) Sirtinol affects cell division in meristems. To analyze cell division, CyclinB1;1:CDB-GUS marker seedlings were grown on sirtinol containing medium and staining for GUS was performed at 2 dag. Scale bar: 50 μm. Black arrows indicate SAM, brackets indicate RAM and bold red arrow marks root-shoot junction.