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. 2017 Feb 14;7:42450. doi: 10.1038/srep42450

Figure 4. Effect of sirtinol on auxin accumulation and PINs expression.

Figure 4

(a) Sirtinol treatment causes ectopic expression of DR5rev:GFP reporter, which marks auxin accumulation. To analyze the effect on auxin accumulation, seeds of DR5rev:GFP reporter line was grown on 10 μM sirtinol and GFP fluorescence was observed in seedlings at 2 dag. Scale bar 50 μm. White dotted lines indicate a shift in domain of DR5rev:GFP expression, in comparison to control. Inset in (a) shows GFP fluorescence analyzed by fluorescence microscope. Bold red arrow marks root-shoot junction. (b–f) Sirtinol alters the expression pattern of PIN genes in root. To analyze the effect on PIN gene expression pattern, PIN2:GUS, PIN3:GUS, PIN4:GUS and PIN7:GUS reporters were grown on 10 μM sirtinol and GUS staining was observed in seedlings at 2 dag. The expression levels of PIN genes were quantified using real time qRT-PCR in 2 days old seedlings. Bold red arrow marks root-shoot junction. Error bars indicate ± SE of three independent experiments. One-way ANOVA was performed for statistical analysis. Asterisks indicate significant statistical differences, ***P < 0.001, **P < 0.01, *P < 0.05. Scale bar 50 μm.