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. 2017 Feb 14;7:42450. doi: 10.1038/srep42450

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Copyright © 2017, The Author(s)

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(a,b) Sirtinol alters the expression of ARR5:GUS and ARR12:GUS, cytokinin signaling markers. Seedlings were grown on 10 μM sirtinol and GUS staining was observed at 2 dag. Bold red arrow marks root-shoot junction. Scale bar: 50 μm. (c) Sirtinol alters the expression level of both cytokinin signaling and biosynthesis genes. Expression level of cytokinin biosynthesis and signaling genes was quantified using real time qRT-PCR in 2 days old sirtinol grown (10 μM) seedlings. Error bars indicate ± SE of three independent experiments. One-way ANOVA was performed for statistical analysis. Asterisks indicate significant statistical differences, ***P < 0.001, **P < 0.01, *P < 0.05.