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. 2017 Feb 14;7:42450. doi: 10.1038/srep42450

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Copyright © 2017, The Author(s)

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(a,b) Sirtinol inhibits primary root growth of wild type plants. To measure root growth, 5 days old wild type seedlings were transferred on sirtinol (5 μM) containing medium and grown vertically. Root length was measured at 0, 1, 3 and 5 dat. One-way ANOVA was performed for statistical analysis. Asterisks indicate significant statistical differences, ***P < 0.001, **P < 0.01, *P < 0.05. Experiment was repeated two times with reproducible results (n = 15). Scale bar: 1 cm. (c) Sirtinol affects LR development of wild type plants. To analyze the LR initiation and growth pattern, 5 days old wild type seedlings were transferred on sirtinol (5 μM) containing medium and LR initiation and growth was observed at 1, 3 and 5 dat. Picture depicts difference in LR development at different regions of root at 5 dat. Scale bar: 1mm. (d) Sirtinol causes defective LR positioning. To analyze the LR positioning defect, 5 days old seedlings were transferred to sirtinol (5 μM) and developing LRPs were marked (arrows) after 1 dat. Scale bar: 50 μm.