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. 2017 Feb 14;7:42450. doi: 10.1038/srep42450

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Copyright © 2017, The Author(s)

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(a,b) Sirtinol affects DR5rev:GFP expression in both primary root and LRs. To analyze the effect on DR5rev:GFP expression, 5 days old seedlings were transferred to sirtinol (5 μM) containing medium and GFP fluorescence was observed at 2 dat. Arrows show developing LRPs. Scale bar: 50 μm. (c,d) Sirtinol affects PIN genes expression in both primary root and LRs. To analyze the effect on PIN2:GUS, PIN3:GUS, PIN4:GUS, and PIN7:GUS expression, 5 days old seedlings were transferred to sirtinol containing medium (5 μM) and GUS expression was observed at 2 dat in primary root and LRs. Scale bar: 50 μm.