Skip to main content
. 2017 Feb 14;7:42473. doi: 10.1038/srep42473

Figure 3. IV/S. aureus super-infection increases IL-6 secretion in Calu-3 and A549 cells.

Figure 3

Calu-3 (a,b) or A549 (a,c) cells were infected with IV H1N1(M) (MOI 5) for 0.5 h and super-infected with S. aureus 6850 (MOI 50). Extracellular bacteria were removed by gentamicin treatment 3 h after bacterial infection. At 8 h p.i. cell supernatants were collected and secreted IL-6 was determined in duplicates by ELISA. One representative experiment (mean + SD) is depicted to show the quantitative amounts of IL-6 (a). Means ± SD of three independent experiments are shown, where IV-infected samples were arbitrarily set as 100% (b,c). After normalisation, two-tailed unpaired t-tests were performed for comparison between IV H1N1(M)-infected and IV H1N1(M)/S. aureus 6850 super-infected samples (*p < 0.05, **p < 0.01).