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. 2017 Feb 5;130(3):309–317. doi: 10.4103/0366-6999.198922

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Copyright: © 2017 Chinese Medical Journal

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Western blotting analysis of cytosolic and mitochondrial fraction of Pim-1, Bcl-2, and Cyt C in myocardium. The Pim-1 (44,000 Da) (a and b) was analyzed by Western blotting with specific Pim-1 antibody at 1 h of reperfusion; the Bcl-2 (26,000 Da) (c and d) was analyzed by Western blotting with specific Bcl-2 antibody at 1 h of reperfusion; and the Cyt C (15,000 Da) (e and f) was analyzed by Western blotting with specific Cyt C antibody at 1 h of reperfusion. (n = 3 for each group). Data were reported as mean ± SD. *P < 0.05 versus CON; ^†P < 0.05 versus I/R; ^‡P < 0.05 versus Sev (repeated measures ANOVA). CON: Control group; I/R: Ischemia/reperfusion; Sev: Sevoflurane postconditioning; Sev+PIM-Inh: Sevoflurane postconditioning + Pim-1 inhibitor II; Sev+DMSO: Sevoflurane postconditioning + dimethyl sulfoxide; PIM-Inh: Pim-1 inhibitor II; Pim-1: Proto-oncogene serine/threonine-protein kinase 1; Bcl-2: B-cell leukemia/lymphoma 2; Cyt C: Cytochrome C; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; COX-IV: Cytochrome C oxidase IV; SD: Standard deviation; ANOVA: Analysis of variance.