Table 4.
Semi-quantitative evaluation of expression of EphrinB2 and OPG in the cortical defects after immunohistochemical staining
| Groups (n = 3) | EphrinB2 | OPG | ||||||
|---|---|---|---|---|---|---|---|---|
| Day 3 | Day 7 | Day 14 | Day 28 | Day 3 | Day 7 | Day 14 | Day 28 | |
| S | 7.47 ± 0.46 | 9.24 ± 0.22 | 11.99 ± 0.54 | 9.58 ± 0.31 | 6.37 ± 0.29 | 5.37 ± 0.17 | 3.41 ± 0.45 | 1.50 ± 0.12 |
| K | 8.66 ± 0.10 | 11.71 ± 0.06 | 10.03 ± 0.67 | 5.75 ± 0.29 | 5.46 ± 0.51 | 5.60 ± 0.33 | 4.48 ± 0.30 | 2.53 ± 0.19 |
| F | 12.42 ± 0.80* | 14.11 ± 0.66* | 13.92 ± 0.79 | 11.65 ± 0.67† | 7.52 ± 0.33 | 8.95 ± 0.06 | 8.12 ± 0.11* | 3.73 ± 0.33† |
| N | 18.14 ± 0.45*,† | 21.57 ± 0.54*,†,‡ | 19.05 ± 0.70*,† | 16.7 ± 0.74*,† | 9.56 ± 0.44*,† | 13.67 ± 0.42*,†,‡ | 11.28 ± 0.33*,†,‡ | 5.61 ± 0.48*,† |
Three sections from each defect were obtained on days 3, 7, 14, and 28. The positive area of immunostaining was calculated automatically and presented as a percentage of the total cortical defect area. Values represent the mean ± SD of three sections. *P<0.05 versus groups S; †P<0.05 versus groups K; ‡P<0.05 versus groups F. OPG: Osteoprotegerin; S: Saline; K: Empty nanocapsules; F: Free 15-Deoxy-△12,14-prostaglandin J2; N: 15-Deoxy-△12,14-prostaglandin J2 nanocapsules; SD: Standard deviation.