Fig. 1.

Expression of inducible, mutated cMyBP-C protein. a Schematic diagram showing domain organization and sequences of wild-type cMyBP-C (Wt) and cMyBP-C^S2− (m domain; R266, R270, R271 mutated to alanine). b Expression of different lines of the S2- transgene in the S2-xtTA mouse hearts. Representative Western blots using anti-cMyBP-C and anti-c-myc antibodies. α-Sarcomeric actin was used as a loading control. c Western blot analyses of cMyBP-C levels in the heart. To obtain complete replacement of endogenous cMyBP-C with the Tg mutant proteins, the individual cMyBP-C^S2−-xtTA double Tg lines were bred into the cMyBP-C^−/− background. Transgenic expression of the cMyBP-C^S2− protein approximated normal levels of the protein in the Ntg hearts. d Quantitation of transgene expression via Western blotting confirmed equivalent expression of cMyBP-C^S2− protein relative to normal cMyBP-C protein in the Ntg hearts