Figure 5.

MCS invasion. A. MCS embedded in 3D collagen gels. Bright field images showing example spheroids for the indicated cell lines are shown upon initial plating (0 time) and 48 hours after plating. Data represents > 10 spheroids, over > 4 individual experiments. B. Merged image of phalloidin (red) and Hoechst Blue (blue) stained SK-N-SH MCS 48 hours after plating. Lower panel shows magnified image of boxed region in the top panel. Arrow points to collectively invading island of cells, asterisks highlight examples of individually invading cells. C-E. Representative time-lapse montages of MCS from the indicated cell lines over a 24 hour time period. Colored images on the right-hand side display paths traced by individual cells as they invade away from the MCS (refer to Supplementary movie 1 to see the paths traced over time). (C) Arrows indicate cells displaying the typical invasion morphology of the long leading edge and rounded cell body, asterisks indicate paused polygonal-shaped cells. (D) Arrows point to highly round cells and asterisks highlight polygonal-shaped cells. (E) Arrow heads in 8 hr panel indicate the first individual, elongated leader cells to appear. Arrows indicate individual elongated cells. The area between white circular lines indicates area of collective migration. Representative spheroids for all cell lines were selected from > 5 spheroids over 3 individual experiments.