Figure 6.
Expression profiling of cell junction-related genes of BJhTERT fibroblasts before and after coculture with normal and cancer prostate epithelial cells. (A) RT2 Profiler™ PCR Array Human Cell Junction Pathway Finder analysis. The relative expression levels for each gene in fibroblasts after co-culture with PNT2 cells (Group 1) or PC3 cells (Group 2) are plotted against the same gene from the control fibroblasts (Control Group). The middle line shows the similar expression in both groups with 2-fold change boundaries. Genes upregulated >2-fold lie above the middle line and the downregulated genes lie below the line. (B) Gene expression levels for fibroblasts, PNT2- and PC3-confronted fibroblasts for junctions pathways with highest expression levels. (C) Immunostaining for a-smooth muscle actin (SMA) (green). Nuclear counterstain - DAPI (blue).