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. 2016 May 9;11(1):24–38. doi: 10.1080/19336918.2016.1178449

Figure 2.

Figure 2.

Expression of Fzd5 and Ryk receptors in HCAEC. Expression of mRNA levels of (A) Fzd5 and (B) Ryk in HCAEC treated with Wnt5A and IL-1β (IL-1b) for 8 h as outlined in Methods. Data were obtained from 3 independent qRT-PCR experiments with duplicate samples and expressed as the mean ± SEM. **P < 0.01, IL-1β versus untreated. (C) Quantitative ratio of constitutive levels of Fzd5 and Ryk mRNAs in HCAEC. ***P < 0.001, Ryk vs. Fzd5 by Student's t-test. (D) Protein expression of Fzd5 and Ryk receptors in HCAEC either untreated (left panel) or treated with IL-1β (IL-1) for 24 h. Images show immunofluorescence staining using specific antibodies for Fzd5 and Ryk (red), F-actin (phalloidin, green) and nuclei (DAPI, blue). Zeiss Axioskope, original magnification 200x.