Figure 5.

ECIS barrier function assays in HCAEC. Uniform confluent monolayers of HCAEC cultured in stabilized and collagen coated 8W10E+ ECIS culture chambers (see Methods and Figure 5 of Online Supplement) were treated with Wnt5A and IL-1β. Resistance of HCAEC monolayers was continuously recorded in Ohms every 5 min at multiple frequencies ranging from 62.6 Hz to 64 kHz, normalized to its value at time zero, and plotted with respect to time. Resistance measured from duplicate wells were grouped and averaged to plot as a single curve with error bars representing the SD. Data shown are the resistance measurements conducted at 4000 Hz from 4 independent experiments. (A) Un transfected HCAEC. Black, Untreated; Green, Wnt5A; Purple, IL-1β. (B) Ryk siRNA transfected HCAEC. Black, Untreated; Green, Wnt5A; Purple, IL-1β.