Figure 2.

Results of the AFM and live cell imaging measurements for the 3 glioblastoma cell lines. (A) Shows the mean measured Young´s modulus with standard error of the mean (SEM) for each cell line and treatment. Statistical significance was achieved for treatments of U138 (n_CTL = 60; n_JWH-133 = 40; n_ACEA = 40) cells only. U87 (n_CTL = 60; n_JWH-133 = 60; n_ACEA = 40) and LN229 (n_CTL = 60; n_JWH-133 = 40; n_ACEA = 40) cells did not show a change in elasticity. No receptor/agonist specific effect could be observed. (B) Calculated mean adhesion energies to the cantilever via the DMT model. All treatments resulted in a decrease of the adhesiveness for all cell lines, except for U87 treated with ACEA. The numbers of analyzed cells used for determination of adhesion energy were identical to those for the Young´s modulus. (C) Derived speeds (mean ± SEM) from the time lapse images. One can observe diverse significant effects on the measured speed, with no apparent receptor/agonist specificity (U138: n_CTL = 67, n_JWH-133 = 81, n_ACEA = 78. For LN229: n_CTL = 118, n_JWH-133 = 177, n_ACEA = 57. For U87: n_CTL = 158, n_JWH-133 = 230, n_ACEA = 93.). (D) Mean area and SEM of each cell line treated with cannabinoids or as control measurement. The CB2 agonist led to a decrease of the area for U87 and LN229 cells but had no effect on the U138 cells. The CB1 agonist only resulted in a decrease of area for the U87 cells. No receptor/agonist specificity that holds for all cells could be observed. The numbers of experimental values for the cell area were identical to the ones for the cell speed. Statistics was performed using the Mann-Whitney test and significance was chosen for p < 0.05. The asterisk denotes significant results regarding the control measurement of the same cell line.