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. 2016 Dec 26;9(1):7. doi: 10.3390/toxins9010007

Figure 2.

Figure 2

Identification of linear and refolded µ-TRTX-Hl1a; (A) the linear µ-TRTX-Hl1a was analyzed by C18 RP-HPLC. The elution was performed with the gradients of acetonitrile in 0.1% (v/v) trifluoroacetic acid in water at a flow rate of 1 mL/min. The gradient of acetonitrile was increased from 10% to 50% during the time of 10 to 50 min; (B) MALDI-TOF-MS determination of the molecular weight of linear µ-TRTX-Hl1a; (C) the refolded µ-TRTX-Hl1a was purified on C18 RP-HPLC. The elution was performed with the gradients of acetonitrile in 0.1% (v/v) trifluoroacetic acid in water at a flow rate of 1 mL/min. The gradient of acetonitrile was increased from 10% to 50% during the time of 10 to 50 min; (D) MALDI-TOF-MS determination of the molecular weight of refolded µ-TRTX-Hl1a.