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. 2016 Dec 26;9(1):7. doi: 10.3390/toxins9010007

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of µ-TRTX-Hl1a on Nav1.8 expressed in ND7/23 cells; (A) current traces were evoked by a 50-ms step depolarization to −10 mV from a holding potential of −80 mV every 5 s. The effect of 2 μM µ-TRTX-Hl1a on the currents of Na_V1.8; (B) dose-dependent inhibition of µ-TRTX-Hl1a on Na_V1.8 channel; (C) the current-voltage (I-V) relationship for Na_V1.8 channel currents before (solid circles) and after (open circles) application of 2 µM µ-TRTX-Hl1a; (D) the conductance-voltage (G-V) relationship for Na_V1.8 channel before (solid circles) and after (open circles) treatment of 2 μM µ-TRTX-Hl1a (n = 5); and (E) steady-state inactivation of Na_V1.8 channel currents before (solid circles) and after (open circles) application of 10 μM µ-TRTX-Hl1a (n = 5).