Table 1.
Buffer condition | Temperature (℃) | Duration (h) | pH | Folding Yield % |
---|---|---|---|---|
0.1 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.3 mmol/L GSSG, 3 mmol/L GSH | 25 | 24 | 7.2 | 6.04 |
4 | 24 | 7.2 | 7.08 | |
4 | 24 | 6.8 | 8.52 | |
4 | 48 | 6.8 | 8.46 | |
0.1 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.3 mmol/L GSSG, 3 mmol/L GSH, 1 mol/L L-Arg | 4 | 24 | 7.6 | 8.13 |
4 | 24 | 7.4 | 8.53 | |
4 | 24 | 7.2 | 9.39 | |
4 | 24 | 6.8 | 10.38 | |
0.05 mol/L Tris-HCl, 0.05 mol/L NaCl, 0.15 mmol/L GSSG, 1.5 mmol/L GSH | 4 | 24 | 7.2 | 10.20 |
4 | 24 | 6.8 | 13.46 |