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. 2016 Dec 26;9(1):7. doi: 10.3390/toxins9010007

Table 1.

Refold of µ-TRTX-Hl1a at different condition.

Buffer condition Temperature (℃) Duration (h) pH Folding Yield %
0.1 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.3 mmol/L GSSG, 3 mmol/L GSH 25 24 7.2 6.04
4 24 7.2 7.08
4 24 6.8 8.52
4 48 6.8 8.46
0.1 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.3 mmol/L GSSG, 3 mmol/L GSH, 1 mol/L L-Arg 4 24 7.6 8.13
4 24 7.4 8.53
4 24 7.2 9.39
4 24 6.8 10.38
0.05 mol/L Tris-HCl, 0.05 mol/L NaCl, 0.15 mmol/L GSSG, 1.5 mmol/L GSH 4 24 7.2 10.20
4 24 6.8 13.46