Table 1.

Induction factors produced by samples in the SOS Chromotest.

Induction Factor ± Standard Deviation
Concentration (%)	2-Aminoanthracene a	Positive Control b	Degraded Sample c	Negative Control d
100	3.49 ± 0.08	2.53 ± 0.06	1.06 ± 0.00	1.09 ± 0.05
50	3.02 ± 0.06	1.73 ± 0.14	1.02 ± 0.03	1.12 ± 0.10
25	2.29 ± 0.09	1.08 ± 0.06	1.00 ± 0.01	1.14 ± 0.09
12.5	1.78 ± 0.05	1.18 ± 0.09	1.02 ± 0.01	1.13 ± 0.04
6.25	1.42 ± 0.12	1.04 ± 0.05	0.96 ± 0.06	0.98 ± 0.09
3.125	1.45 ± 0.07	1.07 ± 0.08	1.07 ± 0.03	1.01 ± 0.11

^a Six two-fold dilutions of 2-aminoanthracene (initial concentration = 100 µg/mL) were prepared in 10% dimethyl sulphoxide/saline. A 10-µL volume of diluted 2-aminoanthracene was used as a positive S9 control. ^b A 0.06-mL volume of AFB₁ solution (50 mg/L) was added to 1.44 mL of NB to obtain a final concentration of 2 mg/L. After incubation in the dark at 37 °C for 72 h, six two-fold dilutions were prepared, and a 10-µL volume was used as a positive control. ^c A 0.06-mL volume of AFB₁ solution (50 mg/L) was added to 1.44 mL of culture supernatant of isolate L7 to obtain a final concentration of 2 mg/L. After incubation in the dark at 37 °C for 72 h (AFB₁ degradation ratio = 77.9% ± 2.3%), six two-fold dilutions were prepared, and a 10-µL volume was used as the degraded sample. ^d A 0.06-mL volume of methanol was added to 1.44 mL of culture supernatant of isolate L7. After incubation in the dark at 37 °C for 72 h, six two-fold dilutions were prepared, and a 10-µL volume was used as a negative control.