Table 3.
Summary of BADE purification.
| Purification Step | Total Protein (mg) × 10−3 | Total AFB1 Degradation Activity (U) * | Specific Activity (U·mg−1) × 102 * | Purification (Fold) | Yield (%) |
|---|---|---|---|---|---|
| Culture filtrate | 96.55 | 38.95 | 4.03 | 1.00 | 100.00 |
| Peak 1 (0–20 mL) | 1.35 | 3.55 | 26.30 | 6.53 | 9.10 |
| Peak 2 (40–50 mL) | 4.04 | 15.55 | 38.49 | 9.55 | 39.92 |
| Peak 2 (50–80 mL) | 6.37 | 15.21 | 23.88 | 5.93 | 39.05 |
| Peak 3 (80–95 mL) | 3.26 | 5.63 | 17.27 | 4.29 | 14.45 |
| Peak 3 (95–100 mL) | 0.20 | 5.5 × 10−5 | 0.0028 | 0.00069 | 0.00014 |
* One unit (U) of enzyme activity was defined as the amount of enzyme required to decrease the amount of AFB1 by 1 ng in 72 h at 37 °C.