Fig. 3. Analyzing the dual binding domains of CBSA for target-induced assembly. (A) We generated multiple derivatives of CBSA-5325, including split aptamers with a single binding pocket (LSA) and a pair of point-mutants (CBSA-M1 and CBSA-M2) with sequence alterations that disrupt either of the two binding domains (pink circle). (B) ATMND quenching in the presence or absence of 250 μM cocaine. Quenching was calculated by (F A – F)/F A × 100%, where F A is the fluorescence of 200 nM ATMND in 1× binding buffer alone and F is the fluorescence of the ATMND–CBSA mixture with 250 μM cocaine or without cocaine, respectively.
