Fig. 8. (a) Anaesthetised larval zebrafish incubated with 1 (100 nM) for 24 hours under UV light (left) and 3D micrographs (thickness = 356.2 μm) from two-photon confocal microscopy co-stained with DAPI, Syto9 and propidium iodide. (b) Fluorescence intensity analysis of the uptake of 1 in larval zebrafish brain and retina at 4 and 24 hours. (c) Whole brain region imaging of stained larval zebrafish by 1 co-stained with DAPI, insert: DIC tile scanning of the imaged larval zebrafish. (d) Brain sections of stained adult zebrafish by 1 co-stained with DAPI. (e) Ex vivo assessment of 1 following i.v. injection in mice co-labeled with DAPI and Alexa488-lectin, and zoom in (200×) micrographs showing the detail of the brain capillaries imaged by confocal laser scanning microscopy, with strong internalization of 1 by the brain endothelium and CNS cells at the nuclear and plasma membrane. The scale bar represents 200 μm. Error bars: SEM, n = 3. Abbreviations: Re = retina, B = brain, J = jaw, FI = fin.