Figure 4.

Longitudinal assessment of intracellular lifetime of water and cell size in mice exposed to TAC. A) Mice were imaged at approximately 2 weeks after transverse aortic constriction, and followed-up for up to 46 days before harvesting of the heart. Over this time the intracellular lifetime increased significantly (0.0581 s/week; P=0.0002) as illustrated by the solid line, calculated with a linear mixed effects (LME) model. Dashed lines connect repeated measurements within the same mouse. B) The volume-to-surface ratio calculated from the histologic major and minor cell diameters showed a significant association with the time between TAC and histological examination (0.79±0.18 μm/week; P=0.001). The solid line represents the linear regression line estimate for all mice, based on the fixed effects in the LME model.