Figure 3.

Cell survival was assessed by CCK-8 assay.

Notes: (A) The growth of C-33A cells was markedly obstructed by ARHGAP1 overexpression in a time-dependent manner. (B) The mean cell proliferation rates of C-33A cells that were transfected with lenti-ARHGAP1 for 72 h were significantly weakened. (C) The growth of SiHa cells was markedly obstructed by ARHGAP1 overexpression in a time-dependent manner. (D) The mean cell proliferation rates of SiHa cells that were transfected with lenti-ARHGAP1 for 72 h were significantly weakened. (E) The growth of HeLa cells was markedly increased by ARHGAP1 knockdown in a time-dependent manner. (F) The mean cell proliferation rates of HeLa cells that were transfected with lenti-shRNA-ARHGAP1 for 72 h were significantly increased. n=3, mean ± SD. ***P<0.001 vs control groups.

Abbreviations: CCK-8, Cell Counting Kit-8; OD, optical density; lenti-ARHGAP1, lentivirus targeting ARHGAP1; NC, negative control; shNC, shRNA-NC.