Table 1.
Primers used in qRT-PCR analysis
| Gene | Primer sequence | Species | Amplicon size (bp) |
|---|---|---|---|
| ARHGAP1 | Forward: 5′-CAGCGTGTGATGGAATCAG-3′ Reverse: 5′-TGGAGGAAGGAGCCATAAG-3′ |
Human | 179 |
| MMP2 | Forward: 5′-TTGACGGTAAGGACGGACTC-3′ Reverse: 5′-GGCGTTCCCATACTTCACAC-3′ |
Human | 134 |
| ZEB1 | Forward: 5′-GCTGTAAGTGCCATTTCTC-3′ Reverse: 5′-GTCCCAGTGTTTCAGTTTC-3′ |
Human | 159 |
| CCNB1 | Forward: 5′-CTCCGGTGTTCTGCTTCTC-3′ Reverse: 5′-GCTGTTCTTGGCCTCAGTC-3′ |
Human | 183 |
| Twist | Forward: 5′-AGTCCGCAGTCTTACGAG-3′ Reverse: 5′-GCTTGCCATCTTGGAGTC-3′ |
Human | 228 |
| PCNA | Forward: 5′-GCCTGACAAATGCTTGCTGAC-3′ Reverse: 5′-TTGAGTGCCTCCAACACCTTC-3′ |
Human | 223 |
| GAPDH | Forward: 5′-CACCCACTCCTCCACCTTTG-3′ Reverse: 5′-CCACCACCCTGTTGCTGTAG-3′ |
Human | 110 |
Abbreviations: CCNB1, Cyclin B1; MMP2, matrix metallopeptidase 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCNA, proliferating cell nuclear antigen; qRT-PCR, quantitative real-time polymerase chain reaction; Twist, twist family bHLH transcription factor 1; ZEB1, zinc finger E-box binding homeobox 1.