Figure 1.

Kindlin gene and protein expression levels in human osteosarcoma tissues.

Notes: (A) Kindlin-1 and Kindlin-2 mRNA levels in osteosarcoma tissues were both significantly higher than those in adjacent noncancerous tissues (both P<0.01), whereas Kindlin-3 mRNA levels in osteosarcoma tissues were significantly lower than those in adjacent noncancerous tissues (P<0.05). (B) Kindlin-1 protein levels in osteosarcoma tissues were significantly higher than those in adjacent noncancerous tissues (P<0.01). (C) Kindlin-2 protein levels in osteosarcoma tissues were significantly higher than those in adjacent noncancerous tissues (P<0.01). (D) Kindlin-3 protein levels in osteosarcoma tissues were significantly lower than those in adjacent noncancerous tissues (P<0.01). The mRNA and protein levels of Kindlins were detected by quantitative real-time PCR and Western blot analyses. T and N refer to osteosarcoma and adjacent noncancerous tissues, respectively.

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction.