Table 2. Characteristics of biological variability for concentrations of PON activities measured in follicular fluid (FF) and serum by PON1 Q192R phenotype among in vitro fertilization patients.
| PON Q192R Phenotype | n | σ2T | %σ2B | %σ2F | %σ2A | II | k | CV% | ICC | 95% CI | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| FF | |||||||||||
| Overall | |||||||||||
| Arylesterase (kIU/L) | 118 | 0.132 | 79.29 | 19.28 | 1.44 | 0.26 | 1 | 7.90 | 0.79 | 0.75 | 0.84 |
| Paraoxonase (IU/L) | 118 | 0.254 | 89.45 | 9.80 | 0.75 | 0.12 | 1 | 11.51 | 0.89 | 0.87 | 0.92 |
| Arylesterase (kIU/L) | 48 | 0.122 | 81.43 | 16.80 | 1.77 a | 0.23 | 1 | 7.58 | 0.81 | 0.75 | 0.88 |
| Paraoxonase (IU/L) | 48 | 0.111 | 75.26 | 21.58 a | 3.16 a | 0.33 | 1 | 8.52 | 0.75 | 0.67 | 0.84 |
| QR | |||||||||||
| Arylesterase (kIU/L) | 48 | 0.121 | 77.12 | 21.48 | 1.39 a, b | 0.30 | 1 | 7.46 | 0.77 | 0.69 | 0.85 |
| Paraoxonase (IU/L) | 48 | 0.076 | 75.68 | 23.08 a | 1.24 b | 0.32 | 1 | 6.02 | 0.76 | 0.67 | 0.84 |
| RR | |||||||||||
| Arylesterase (kIU/L) | 22 | 0.175 | 78.71 | 20.25 | 1.04 b | 0.27 | 1 | 9.31 | 0.79 | 0.67 | 0.90 |
| Paraoxonase (IU/L) | 22 | 0.076 | 43.40 | 56.04 b | 0.56 c | 1.30 | 1 | 5.58 | 0.43 | 0.19 | 0.68 |
| Serum | |||||||||||
| Overall | |||||||||||
| Arylesterase (kIU/L) | 157 | 0.092 | 98.91 | − | 1.09 | − | 1 | 5.95 | 0.99 | 0.99 | 0.99 |
| Paraoxonase (IU/L) | 157 | 0.282 | 99.84 | − | 0.16 | − | 1 | 11.08 | 1.00 | 1.00 | 1.00 |
| Arylesterase (kIU/L) | 62 | 0.065 | 98.38 | − | 1.62 a | − | 1 | 4.95 | 0.98 | 0.98 | 0.99 |
| Paraoxonase (IU/L) | 62 | 0.093 | 99.14 | − | 0.86 a | − | 1 | 7.06 | 0.99 | 0.99 | 1.00 |
| QR | |||||||||||
| Arylesterase (kIU/L) | 70 | 0.093 | 99.10 | − | 0.90 b | − | 1 | 6.02 | 0.99 | 0.99 | 1.00 |
| Paraoxonase (IU/L) | 70 | 0.140 | 99.79 | − | 0.21 b | − | 1 | 7.47 | 1.00 | 1.00 | 1.00 |
| RR | |||||||||||
| Arylesterase (kIU/L) | 25 | 0.139 | 99.06 | − | 0.94 b | − | 1 | 7.52 | 0.99 | 0.98 | 1.00 |
| Paraoxonase (IU/L) | 25 | 0.097 | 99.89 | − | 0.11 c | − | 1 | 5.78 | 1.00 | 1.00 | 1.00 |
NOTE: All values were natural log-transformed for the analyses and only women with no missing values were used (i.e., balanced data).
a, b, c Different superscript letters indicate P < 0.05 for difference in and values between phenotypes for arylesterase or paraoxonase activities. For example, in FF paraoxonase activity for RR phenotype is significantly different (P < 0.05) from QQ and QR phenotypes.
CI, confidence interval; CV, coefficient of variation; ICC, intraclass correlation coefficient; II, index of individuality; k, minimum number of specimens required to estimate the women-specific mean value within 10% of the true value; , variability attributed to analytic factors; , variability between-women; , variability between-follicles; , total variability.