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. 2016 Aug 17;7(38):60776–60792. doi: 10.18632/oncotarget.11320

Figure 2. Llgl1 regulates expression of cell lineage markers.

Figure 2

A.-D., I.-L. Cells were incubated with the indicated cell lineage marker and sorted by FACS. A.-D. MCF12A shControl vs shLlgl1 (under normal growth conditions) were incubated with anti-CD49f-PE, anti-CD44-APC, and/or anti-CD24-FITC. E. and F. MCF12A shLlgl1 were sorted based on CD44/CD49f expression, CD444hi/CD49flo (E) and CD44lo/CD49fhi (F). G. Protein lysates were isolated and analyzed by immunoblot using anti-Llgl1 and anti-βactin antibodies. H. Protein lysates were analyzed by immunoblot using anti-Integrin α6 and anti-βactin. (I-L) MCF10A shControl vs shLlgl1 (under normal growth conditions) were incubated with anti-CD49f-PE, anti-CD44-APC, and/or anti-CD24-FITC.