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. 2016 Aug 27;7(38):60885–60895. doi: 10.18632/oncotarget.11659

Figure 2. Compromised calcium influx induced by BCR crosslinking in Stim1&2 DKO B cells.

Figure 2

A. Calcium mobilization monitored by Indo-1 AM imaging. B cells purified from spleen were stimulated with 10μg/ml anti-IgM F(ab)2 for 300 seconds followed by 500nM Ionomycin. B. Gating strategy for immature B cells, marginal zone B cells and mature follicular B cells for calcium influx monitoring on FACS depicted in Fig.2 C. D. E. Calcium influx in immature B cells (C); mature follicular B cells (D) and marginal zone B cells (E) in spleen from mbCre+ control, Stim1 f/f, mb1Cre+, Stim2 f/f, mb1Cre+ and Stim1&2 f/f, mb1Cre+ (DKO) mice.