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A. Proliferation of control or Stim1&2 DKO B cells stimulated with TLR9 ligand CpG, TLR4 ligand LPS and anti-IgM F(ab)2 for 72 hours. B. Class switch in control and Stim1&2 DKO B cells stimulated with LPS or LPS + IL-4 into IgG3 and IgG1. C. Serum level of immunoglobulins IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA in control mb1Cre+, Stim1&2 DKO and Stim2 f/f (control as well) mice.
