Figure 4. Water restriction causes severe dehydration of Vhl-deficient mice and there are no alterations in AQP2 or NKCC2 localization.

A. Urine volume per hour and B. food intake per hour of mice during water restriction test. Vhl^Δ/Δ mice and control littermates (Vhl^fl/fl) were housed in metabolic cages and either provided with powder chow and water ad libitum for 24 h (Day 1 and Day 3) or with wet food (water restriction). Experiment was aborted after 5 h due to severe dehydration of Vhl^Δ/Δ mice. C. Immunofluoresence staining of NKCC2 (red) and DAPI (blue) in thick ascending limb of Henle of unchallenged Vhl^Δ/Δ mice and controls. D. Immunofluoresence staining of Aquaporin 2 (green) and DAPI (blue) in renal collecting duct of unchallenged Vhl^Δ/Δ mice and controls. Scale bars = 25 μm.