Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 7;7(38):61021–61035. doi: 10.18632/oncotarget.10477

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2016 Carino et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

A-B. Gastric cancer cells were serum starved and then primed with TLCA (1, 10 and 100 μM), TDCA (1, 10 and 100 μM), 6-ECDCA (1, 10 and 50 μM) for 72 hours. (A) A scratch wound healing assay is shown: MKN45 cell line left untreated or stimulated with 100 μM CA, CDCA, TLCA, TDCA and 50 μM 6E-CDCA. The images show cell migration after 72 hours of incubation with the indicated compound. After treatments with indicated compounds, cell monolayers were scraped in a straight line using a p200 pipette tip in order to create a “scratch”. A wound was generated and imaged at 0 an 24 hours with a phase-contrast. Images obtained from each samples at both time points were analyzed using Image J software and migration areas were expressed in pixels. All experiments were performed at least in triplicate. *P<0.05 versus not treated (NT).