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. 2016 Jul 7;7(38):61021–61035. doi: 10.18632/oncotarget.10477

Figure 4. GPBAR1 agonists induce a EMT phenotype in MKN45 cells.

Figure 4

A-C. MKN45 cells were plated and after 24 hours of starvation were stimulated for 72 hours with TLCA and TDCA (100 μM). Exposing MKN45 cells to TLCA resulted in 50% percent reduction of E-cadherin mRNA (n= 4; P<0.05) (A), ≈ 100% increase of N-cadherin (n=4; P<0.05) (B) and ≈ 30% increase of vimentin (C). RT-PCR analysis of expression of E- (D) and N-cadherin (F) and vimentin (E) genes in gastric cancers (see figure 1). N-cadherin mRNA expression was found to be significantly higher in patients with advanced gastric cancers (stage III and IV)(p<0.05 versus stage I and II). (F inset) Correlation between N-Cadherin and GPBAR1 mRNA in specimens of human gastric cancers (n= 35 patients; r=0.52, p<0.001). Values are normalized to GAPDH and are expressed relative to those of positive controls, which are arbitrarily settled to 1. The relative mRNA expression is expressed as 2(−ΔΔCt).