Figure 5. Gene array analysis of the expression of genes related to tumor metastasis in MKN45 cells exposed to selective and non-selective GPBAr1 ligands.

The cDNA used for the array was obtained from MKN45 cells (1×10⁶) plated, serum starved for 24 hours and then treated for 72 hours with 6-ECDCA 50μM. 6-ECDCA modulated the expression of 29 genes (18 upregulated and 11 downregulated). A comparative analysis of effects exerted by the three GPBAR1 agonists (Supplementary Figure S2 and S3), demonstrated that the three agents shared a regulatory effect on a small number of genes. Array analysis was carried out with the online software RT2 Profiler PCR Array Data Analysis (http://pcrdataanalysis.sabiosciences.com/pcr/arrayanalysis.php), Up-regulated/down-regulated genes were those genes whose expressions had been altered by more than 1.9 fold.