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. 2016 Jul 7;7(38):61021–61035. doi: 10.18632/oncotarget.10477

Figure 7. GPBAR1 activation enhances the metastatic potential of MKN45 cells.

Figure 7

A-C. MKN45 cells were pre-incubated with 100 μM TLCA and the injected i.p. in NOD-SCID mice (as described in materials and methods). TLCA pretreatment enhanced significantly the number and the volume of peritoneal nodules in comparison to control cells, Cetuximab reverted this pattern. *P<0.05 versus not treated; # P<0.05 versus TLCA. (A-C). Ematoxylin and eosin staining of samples obtained from peritoneal nodules showing extensive necrosis in nodules exposed to cetuximab (D). E. RT-PCR analysis of RNA extracted from peritoneal nodules showing a potent upregulation of ITGB3 in MKN45 cells pre-exposed to TLCA. Values are normalized to GAPDH and are expressed relative to those of positive controls, which are arbitrarily settled to 1. The relative mRNA expression is expressed as 2(−ΔΔCt). *P<0.05 versus control.