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. 2016 Aug 12;7(38):61121–61135. doi: 10.18632/oncotarget.11244

Figure 3. LAL deficiency reduced synthesis and secretion of tumor-promoting cytokines and chemokines in MSCs.

Figure 3

(A) The secretions of IL-6, MCP-1, and IL-10 in lal +/+ or lal −/− FVB/N MSCs′ conditioned medium by ELISA analysis. MSCs were seeded at a density of 5 × 105 cells/well in 6-well plates. Three days later, the conditioned medium was harvested for ELISA analysis. (B) The secretions of IL-6, MCP-1, and IL-10 of lal +/+ or lal −/− C57BL/6 MSCs′ conditioned medium by ELISA analysis. For both (A) and (B), data were expressed as mean ± SD; n = 4. *P < 0.05. (C) Real-time PCR analysis of mRNA expression levels of IL-6, MCP-1, and IL-10 in lal +/+ vs. lal −/− MSCs from FVB/N mice. The relative gene expression was normalized to GAPDH mRNA, and analysis was performed by the 2−ΔΔCT method. Data were expressed as mean ± SD; n = 4. *P < 0.05.