Skip to main content
. 2016 Aug 13;7(38):61166–61182. doi: 10.18632/oncotarget.11278

Figure 1. Downregulation of miR-4653-3p and upregulation of miR-660-5p confirmed in recurrent/metastatic lesion, compared to their matched primary lesion in tamoxifen-resistant patients.

Figure 1

Total RNA was extracted from tumor tissues of recurrent/metastatic (R/M) lesions and their matched primary lesions of 6 TAM-resistant patients from the discovery set. Real-time RT-PCR was performed for evaluating levels of (A) miR-3687, (B) miR-4653-3p, (C) miR-144-3p and (D) miR-660-5p. RNU6B served as an internal control for normalization purpose. Fold change was calculated as the mean ratio of normalized miRNA levels in R/M lesions to matched primary lesions. P values were calculated using paired two-sided t test or Wilcoxon signed ranks test (related samples) as appropriate.